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Molecular methods for bacteriuml picture show Outline Microorganisms determinate methods Molecular methods to study bacteria 1 Microorganisms bed all over Represent more than 50% of the biomass on primer secure & pathogenic Extremely diverse (the oldest life stool on earth) Coccus Bacillus Coccobacillus Fusiform bacillus Spirillum spirochaete Vibrio Cultivation as a classical bacteria study method Cultivation of bacteria for: Obtaining pure cultures acknowledgement Enumeration Liquid media Specific (selective) media and harvest-home conditions are required unharmed media Cultivation Can non enumerate both viable and cold Does not represent host-microbe interaction Difficult and time consume (fastidious growing conditions) 2 Low culturability of bacteria Habitat Human gastrointestinal tract1 Seawater2 Freshwater2 Mesotrophic lake2 Unpolluted estuarine waters2 Activated sludge2 Sediments2 Soil2 1 Culturability (%) 10 0.001 0.25 0.1 0.1 1 0.25 0.3 Vaughan et al. Curr. Is.Intest. Microbiol 1, 2000, 1-12 2 Amann, Rudolf I., et al. Microbiological reviews, 59 (1), 1955, 143-169 50 0.

1 1 3 15 A need for methods that circumvent culturing and practice reliable and fast analysis of microbial communities Molecular characterization of microorganisms lineage of molecular(a) markers directly from (environmental) samples for: Identification Enumeration Cellular molecular markers: DNA RNA Proteins Ribosomes consist of RNA 3 bacterial identification utilise 16S rRNA 16S rRNA (1540nt) 23S rRNA (2900nt) 5S rRNA (120nt) Variable region 1 2 3 4 5 6 7 8 9 Present in every bacterial ! cubicle V6 V4 V5 V7 genetically stable V8 V3 >200,000 sequences in databases Variable and conserved regions V1 (identification and find evolutionary exceed between bacteria) V9 V2 16S rRNA folding 16S rRNA is a competent tar sterilize for...If you want to get a wide of the mark essay, order it on our website: OrderEssay.net

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